Dynamic Three-dimensional Micropatterned Coculture Using Photocurable and Chemically Degradable Hydrogels for Stem Cell Differentiation
نویسندگان
چکیده
We report the spatiotemporal control of a 3D coculture by combining photocurable and chemically degradable hydrogels for coculture of embryonic stem (ES) cells with HepG2 cells. ES cells were encapsulated in micropatterned photocurable poly(ethylene glycol) (PEG) hydrogels. The cell-laden PEG hydrogels were then encapsulated in calcium-alginate (Ca-Alg) hydrogel containing HepG2 cells. The ES cells in the PEG hydrogels were then cocultured with the HepG2 cells in the Ca-Alg hydrogel for 4 days. The Ca-Alg hydrogel containing HepG2 cells was degraded by exposure to sodium citrate solution and the HepG2 cells were removed. ES-derived cells were then cultured in differentiation media.
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